| Position | Amino Acid | Site conservation | Chain | Secondary structure | Number of mutations |
| 1 | M | M:99.4% | | | 1 |
| 2 | E | E:98.8% | | | 3 |
| 3 | Q | Q:92.9% | | | 1 |
| 5 | P | P:98.8% | | | 1 |
| 9 | G | G:100.0% | | | 1 |
| 10 | P | P:98.2% | | | 1 |
| 12 | R | R:98.8% | | | 3 |
| 13 | E | E:96.5% | | | 4 |
| 14 | P | P:99.4% | | | 1 |
| 16 | N | N:95.3% | | | 3 |
| 17 | E | E:94.7% | | | 2 |
| 18 | W | W:99.4% | | | 4 |
| 20 | L | L:98.8% | | | 1 |
| 21 | E | E:95.3% | | | 3 |
| 22 | L | L:91.2% | | | 4 |
| 23 | L | L:99.4% | | | 2 |
| 24 | E | E:99.4% | | | 3 |
| 25 | E | E:94.7% | | | 2 |
| 26 | L | L:97.6% | | | 1 |
| 27 | K | K:100.0% | | | 1 |
| 28 | N | N:39.4%,other:60.6% | | | 1 |
| 29 | E | E:100.0% | | | 4 |
| 30 | A | A:98.2% | | | 4 |
| 31 | V | V:98.8% | | | 1 |
| 32 | R | R:96.5% | | | 4 |
| 33 | H | H:98.2% | | | 5 |
| 34 | F | F:97.6% | | | 2 |
| 35 | P | P:98.2% | | | 3 |
| 36 | R | R:97.6% | | | 3 |
| 38 | W | W:96.5% | | | 2 |
| 39 | L | L:100.0% | | | 1 |
| 40 | H | H:93.5% | | | 2 |
| 42 | L | L:97.1% | | | 1 |
| 43 | G | G:99.4% | | | 3 |
| 44 | Q | Q:94.1% | | | 1 |
| 45 | H | H:55.3%,Y:40.0% | | | 3 |
| 47 | Y | Y:100.0% | | | 1 |
| 48 | E | E:32.4%,N:47.6% | | | 2 |
| 50 | Y | Y:100.0% | | | 1 |
| 51 | G | G:98.8% | | | 2 |
| 52 | D | D:99.4% | | | 1 |
| 53 | T | T:98.8% | | | 1 |
| 54 | W | W:98.8% | | | 4 |
| 55 | A | E:63.5%,other:36.5% | | | 1 |
| 57 | V | V:99.4% | | | 1 |
| 58 | E | E:85.9% | | | 1 |
| 59 | A | A:95.3% | | | 2 |
| 60 | I | I:87.6% | | | 2 |
| 61 | I | I:87.1% | | | 1 |
| 62 | R | R:97.1% | | | 4 |
| 63 | I | I:80.0% | | | 6 |
| 64 | L | L:99.4% | | | 7 |
| 65 | Q | Q:98.8% | | | 5 |
| 67 | L | L:97.1% | | | 6 |
| 68 | L | L:94.7% | | | 3 |
| 69 | F | F:100.0% | | | 1 |
| 70 | I | I:66.5%,V:25.3% | | | 4 |
| 71 | H | H:100.0% | | | 5 |
| 72 | F | F:92.9% | | | 2 |
Click on row for detailed mutagenesis information for a residue. All data is mapped to the HXB2 isolate.